Labs Recruiting Graduate Students for Fall 2023
Labs Recruiting Graduate Students for Fall 2023
Applications for graduate school are due December 1. All applicants must fulfill requirements of the MU Graduate School and show likelihood of successfully completing the departmental program. For more information about graduate admission to the Division of Biological Sciences, click on the button below.
The faculty listed below are recruiting graduate students to work on projects in their labs, starting fall 2023. Click on each faculty member's name to read about the lab and projects.
Epithelia are the most common tissue type in animals. They can be found at organ boundaries, where they perform functions including absorption (the intestine), secretion (glands), filtration (kidneys), and gas exchange (the lungs). Epithelia are typically organized into sheets of cells that are one-cell thick, and our lab aims to understand how these sheets develop.
The Finegan Bergstralh Lab is currently working on two problems. 1) How do epithelial cells figure out the right direction in which to divide? 2) What happens when they don’t? These questions sit at the intersection of basic cell and developmental biology, biomedical research, and the physical sciences, so we take an interdisciplinary approach to answering them; our lab members come from several scientific backgrounds and use a range of techniques that includes traditional genetics, protein biochemistry, advanced light microscopy, and computational modelling.
Graduate student projects are tailored to suit student’s individual research interests, expertise, and career goals. We’d love to discuss opportunities for new lab members!
We are relocating to Mizzou in 2023 and are committed to fostering a diverse and welcoming research community.
The Birchler Lab focuses on genetics and genomics of maize with specific interests in developing synthetic chromosomes and new transformation techniques, the molecular basis of genomic balance, the molecular mechanism of the drive mechanism of the supernumerary B chromosome, and the molecular basis of heterosis, using maize as the model organism.
There are currently nine lab members consisting of technicians, postdocs, graduate students, and undergraduates. We have previously established foundational minichromosomes for genetic engineering and are currently developing procedures for gene stacking of individual genes and large segment additions on the minichromosomes. With regard to genomic balance, we are pursuing the molecular basis of why the addition or subtraction of a chromosome has a much more severe effect on an organism than changing the dosage of the whole set of chromosomes. These studies involve RNA-seq and chromatin analyses using the genetic tricks of maize. Our lab was involved in determining the sequence of the B chromosome of maize, which has properties to perpetuate itself despite that fact that it is a dispensable chromosome. CRISPR-Cas9 editing of candidate genes involved in this drive mechanism is being conducted to dissect their function in conjunction with genetics and cytological analyses. Heterosis is the increased biomass and vigor of hybrid plants, which has been used in agricultural practices to improve yield. We are using genetic and genomic tools to gain new insights into the molecular basis.
Students typically choose a project in which they are interested and are encouraged to develop it creatively within the context of the lab.
Brown lab is a research community comprised of research technicians, undergraduate students, graduate students, and postdoctoral scholars. Dr. Brown is committed to investing in the success of her trainees and develops a customized mentoring plan for each individual. We welcome all individuals with an interest in microbial cell biology to learn more our research and lab environment.
Research overview: The Brown lab is interested in understanding the principles that govern bacterial morphology, a readily observable facet of microbial cell biology. Bacteria exhibit an amazing diversity of shapes and sizes that are precisely reproduced at every generation, indicating that morphology plays an important role in the life of these bacteria. Yet, we are still very far having a comprehensive understanding of how bacterial morphologies are generated. The Brown lab uses methods including bacterial genetics, quantitative cell biology, and microscopy to study growth patterning in the bacterial plant pathogen, Agrobacterium tumefaciens.
We invite you to meet some members of the lab and learn a little bit about our research projects by watching our LabBlitz 2020 video: https://youtu.be/KJOIBzMPIhg
I am a molecular geneticist with >30 years of experience in the characterization of genes involved in human disease, the generation and genetic/phenotypic characterization of animal models and the development of various molecular tools for genetic testing of laboratory animals and cell lines. I am the Director of the Rat Resource and Research Center (RRRC), Director of the MU Animal Modeling Core (AMC) and co-Director of the Comparative Medicine Program. My research lab as well as the RRRC and AMC are located in a state-of-the art facility at the Discovery Ridge Research Park. Our team consists of a highly collaborative group that includes junior faculty, veterinarians, graduate students, undergraduates and research technicians. My lab develops and uses genetically modified rodent and zebrafish models to characterize genes and proteins relevant to a variety of diseases including hereditary deafness, polycystic kidney disease, inflammatory bowel disease, Alzheimer’s disease, and epilepsy. In addition, we study the impact of the microbiota and a variety of therapeutic agents/interventions including dietary/probiotic supplementation on disease phenotypes in animal models.
Examples of recent projects include making and characterizing green and red fluorescent rats, generating and characterizing rat models for the study of COVID-19, studying the effect of probiotic supplementation in zebrafish under stress and rats with Alzheimer’s Disease, analyzing the effects of dietary supplementation with plant-derived extracts on physiological measures in a rat model of obesity and diabetes, and developing methods for performing in vitro fertilization using fresh and frozen rat sperm.
Learn more about the Rat Resource and Research Center
My lab investigates the mechanisms that regulate an evolutionarily conserved neuronal migration in the vertebrate brain and the functional consequences of defective migration. We identified the first gene (Vangl2) that controls the initiation of facial branchiomotor neuron migration and discovered the first and only gene so far (Celsr1), that controls the direction of migration. We are currently examining the cellular and molecular mechanisms of how Celsr1 inhibits the function of a chemoattractant Wnt5a to suppress inappropriate neuronal migration in mouse. My lab examines the behavioral consequences of defective migration by evaluating the functional output of the branchiomotor circuits that drive jaw movements in zebrafish. These studies address a long-standing question in the field: does the position of a neuron within a circuit influence its output? Since dendritic inputs could be affected by the position of the neuronal soma, it is inferred that changes in neuronal position will affect circuit output, but this assumption has not been tested. We are using various strategies to rigorously test for a causal link between neuronal position and circuit output. Our studies employ several techniques including CRISPR, live imaging, conditional knockouts, mutant analysis, and biochemical analysis.
There are opportunities for students to work on the mouse or zebrafish project based on their interests. I welcome questions from interested students about our research and projects.
The processes of domestication and breeding have had serious consequences on modern maize, particularly for the genes that are directly responsible for the transitions from the weedy teosinte ancestor to landrace (heirloom) populations to modern corn. These genes are often involved in kernel size and composition, as these traits were targets of selection during domestication and breeding. In addition, the focus on yield as the primary trait of interest in the private sector for the last century has resulted in a loss of variation for food quality traits.
My lab uses a combination of genetic and breeding methodologies to explore and utilize the incredible phenotypic diversity in seed traits in heirloom corns from across the Americas. Ongoing research projects focus on: food quality traits such as flavor, texture, aroma, and nutrition; breeding improved varieties for improved whiskey flavor; increasing anthocyanin and other phenolic compounds in the kernels for antioxidants and natural food dyes; characterization of 1000 heirloom corns from the United States and Canada; and many other projects at the intersection of seed traits and genetically diverse maize germplasm.
The Holliday lab uses anatomical techniques, imaging and 3D biomechanical approaches to explore how animals are built, how they work and how they evolve. We tend to focus on the craniofacial biology and mechanics of reptiles including living species like crocs, birds and lizards, as well as their extinct ancestors like dinosaurs. We are experts in contrast-enhanced microCT, which allows us to see and measure soft tissues like muscles, brains, nerves and cartilages and are developing new methods in visualizing and quantifying complex anatomies of nerves, muscle architecture and joint systems in the skull and limbs, particularly in collaboration with Biology faculty member Kevin Middleton. We also keep a foot in the fossil record, using our understanding of living species to help guide our interpretations of how extinct species lived and how modern clades originated. We have shown how crocodilian skulls and feeding performance evolved, how blood vessels in dinosaur skulls aided in thermal biology, as well as how reconstructing cranial nerves help understand sensory biology in animals like Tyrannosaurus. We also like to migrate and exercise 3D models of anatomy into online and virtual reality environments.
Ongoing projects include:
1) 3D muscle architecture, comparative biomechanics and evolution of vertebrates.
We are in the throes of learning how accurately we can build 3D models of muscle fibers, how we can estimate physiological properties using these data, and how we can incorporate these data into developmental, biomechanical, ecological and evolutionary studies of jaw muscles and appendicular muscles. Notably, we are employing these methods to study the jaw muscles of birds and reptiles, their significance for the diversification of birds, patterns of ecomorphology in lineages of lizards and snakes, as well as in a growing sample of crocodilians and mammals. This work is all done in parallel with studies of skeletal morphology and mechanics so that we can build high fidelity 3D models of musculoskeletal systems to test a variety of evolutionary and ecological hypotheses. We are also using these approaches to study the origin of grasping in primates and other mammals via a collaborative grant with Carol Ward and Kevin Middleton thanks to the Leakey Foundation. Other ideas are welcome, the sky is the limit.
2) Biomechanics and evolution of crocodilians and dinosaurs (inc birds).
We have vested interests in learning how the heads of birds evolved from dinosaurs and how the heads of modern crocs evolved from their ancestors and all of the diversity in between. We spend time visiting museums, CT scanning fossils, and discovering how their joints are built, how their skulls work, and how these lineages have changed over the past 250million years. We’re currently trying to find traces of fossilized cartilage in joints with colleagues in Mizzou’s Geology Dept (Schiffbauer, Selly), understand how mandibular joints work in theropod dinosaurs, how the palate and cranial kinesis evolved along the line to birds and how patterns of somatosenstation and beaks evolved since the beginnings of the Mesozoic.
3) Evolution of mammalian jaw-ear musculature.
We know a lot about how the primitive jaw joint bones of mammal ancestors evolved into ear ossicles now used in hearing, but we know little about how the muscles evolved alongside the bones. We have an internal grant to explore this anatomy in growing sample of young and adult pigs, opossums and primates to better understand muscle-bone connections during the suckling-to-weaning transition with an eye towards getting at muscle evolution across mammals.
4) Biology of ‘exotic’ species.
We collaborate with a number of veterinarian colleagues which leads to a variety of projects that have proximal, medical outcomes, but also that feed into broader ecomorphological studies. Currently, along with several Avian veterinarians, we are using new contrast imaging approaches to study the sensory receptors inside the beaks of birds to help treat malocclusion and avoid aggressive beak trimming. This work will extend into studies of many other bird species, particularly parrots and ducks. We are also working on the anatomy of pangolins with colleagues from Chicago’s Brookfield Zoo.
We currently have 4 PhD students and 1 Undergraduate researcher in the lab. We try to find projects that fit with students’ passions and interests, but also play to our strengths. Everyone helps out with each other’s projects in a collaborative fashion. We work on crocodiles and dinosaurs, so we try our best to have fun and appreciate our jobs, while also doing some wicked science.
The Kang laboratory is a part of the USDA Agricultural Research Service Biological Control of Insects Research Laboratory and is affiliated with the Division of Biological Sciences at the University of Missouri. We investigate the utilization of microbiomes in the control of insect pests. Our interests are the environmental influences and within community interactions of microbiomes, host-microbe interactions, and their downstream impact on host immunity, metabolism, reproduction, and survival. We value diversity and inclusion, honest communication, and aim to foster an environment focused on the development and success of our students.
Our laboratory focuses on a multidisciplinary approach. First, we capture microbial and host variation in the wild. Next, we use statistical modeling to generate hypotheses. We then use the genetically tractable model organism Drosophila melanogaster to test and refine these hypotheses. The knowledge gained from these experiments is then utilized to control agricultural pests, such as the berry pest, Spotted Wing Drosophila (Drosophila suzukii. Current research efforts include
1. Microbial influence on Drosophila suzukiifitness.
- Testing which microbes and what genes they possess are responsible for changes in the host.
- Testing transcriptional changes made in the host by the microbes.
2. Microbial ecology of insect pests
- Computational modeling of the wild Drosophila suzukii microbial assembly.
- Testing microbial priority effects.
- Utilizing artificial intelligence to identify metagenomic factors leading to the persistence of grape sour rot bacteria in the fly host
3. Investigation of the role of prostaglandins in fly innate immune response and resistance to pathogens.
Our ultimate scientific goal is to combine these efforts to enable tailor-made microbial control of agricultural pests and enhance existing methods.
Research in the King Lab addresses fundamental questions in evolutionary genomics, seeking to understand how genomes change when phenotypes evolve. We integrate computational methods with large-scale empirical studies, with a primary focus on understanding the evolution of complex traits, particularly sensory and life history traits, using the fruit fly model system. Current specific research areas include:
Experimental Evolution: We often use the approach of evolving flies under controlled conditions to observe how phenotypes evolve in concert while also tracking the genomic changes that occur during adaptation. For example, we can produce populations of flies adapted to different diets, or populations with extremely high flight performance, or populations that can withstand high temperatures.
Development of Models of Genome-wide Evolution: Past development of models of evolution often either model only a few locations in the genome or focus on how phenotypes evolve without considering specific genomic loci. We are developing models of evolution that track full genomes to produce better predictions for allele frequency changes over time.
Genotype to Phenotype Mapping of Complex Traits: Our lab takes a systems approach using established mapping panels to study several complex phenotypes (e.g., lifespan, fecundity, learning, memory, thermal tolerance, dispersal) with the goal of understanding how traits are interrelated from gene expression to physiological traits to high-level traits.
Our lab culture is centered on inclusion, diversity & equity, anti-racism & anti-oppression, collaboration & community, the well-being of our members, and open science (see https://elizabethking.org/labvalues/ for more info). Training new scientists (broadly defined) is one of the main goals of our lab, and we welcome prospective students interested in either computational or empirical projects.
Saludos y gracias por su interés en unirse a nuestro equipo “Chipojo Lab”. I am a first-generation college student and have been extremely lucky to work with amazing graduate and undergraduate students that have contributed significantly to our research program.
I am a theory-driven behavioral ecologist and have established an integrative and interdisciplinary research program that reflects my interest in developing an intimate understanding of the behavior, ecology, and physiology of organisms under natural conditions, with the ultimate goal of elucidating evolutionary processes.
Research in the Chipojo Lab combines experimental and observational approaches, including long-term manipulations of natural populations. Our work is mostly done with tropical lizards in the genus Anolis. Our findings have greatly contributed to our knowledge of the evolutionary diversity of this group. More generally, our research has changed how we study communication and physiological constraints under natural conditions and how we view the behavioral capacity of reptiles.
Dr. Lei’s research focuses on ovarian reserve formation, maintenance, and its associated ovarian dysfunction and diseases. Oocytes have the unique ability to program early embryogenesis, but during a normal life cycle of a female, only a small number of mature oocytes (eggs) are produced. A drastic decline in egg quality in young/middle-aged women causes a significantly increased chance of infertility and birth defects. The loss of primary oocytes due to genetic mutations, aging and environmental and toxic factors leads to diminished ovarian function and an increased chance of ovarian cancer in postmenopausal women.
The goal of our research is to uncover the mechanisms of mammalian ovarian reserve formation and maintenance and to apply this fundamental knowledge to better understand female ovarian dysfunction and diseases, including premature ovarian insufficiency, infertility and ovarian cancer. Our previous studies revealed that during ovarian reserve formation in mouse fetal ovaries, oocytes differentiate via intercellular transport of organelle & cytoplasm among interconnected sister germ cells. In the differentiated oocyte, enriched organelles organize into a Balbiani body (B-body). The B-body is involved in ovarian reserve maintenance in adult ovaries through RNA storage and translational regulation. Currently, we are investigating in mice and humans: 1) establishment of fetal germ cell connectivity and its role in ovarian reserve formation; 2) how intercellular transport of organelles and cytoplasm during ovarian reserve formation influences ovarian function and female fertility in adulthood; 3) roles of organelle organization and RNA storage in ovarian reserve maintenance and activation in adult females; and 4) cellular origin and microenvironment of ovarian cancer. The long-term goal of our research is to decipher genetic and environmental factor-caused ovarian dysfunction and diseases and to develop novel medical solutions for early detection and prevention of ovarian diseases.
The mission of the Lin Brain Lab is to prevent Alzheimer’s disease (AD) by identifying effective pharmacological and nutritional interventions using neuroimaging, gut microbiome and multi-omics analyses, and machine/deep learning methods. The ultimate goal is to provide individualized solution with precision medicine via gut-brain axis. Dr. Lin is a well-known expert on translational neuroimaging of brain vascular and metabolic function in aging, AD, stroke and traumatic brain injury (TBI). She developed and applied magnetic resonance imaging (MRI) and spectroscopy, and positron emission tomography (PET) to test nutritional and pharmacologic approaches for protecting the brain from aging, TBI and AD. She is also an expert in artificial intelligence (AI) and multi-omics (such as metabolomics and gut microbiome). She has applied AI to identify markers that are highly predictable for AD development and progression, and applied gut microbiome analyses to study gut-brain interaction underlying AD.
Graduate students join the lab will have opportunities to participate in dietary intervention studies with animal models or human participants. Students will work side-by-side with Dr. Lin and other lab members and receive training on applying various technologies, including operating MRI/PET scanners, processing brain imaging, and analyzing multi-metrics data using advanced statistical methods and AI.
The McSteen lab uses a genetic approach to understand plant development. We start by screening for mutants in maize with defects in tassel and ear development and then identify the genes that are mutated and study how they work at the physiological, biochemical, cellular, genomic, genetic, molecular and even evolutionary level. Through this approach, we have identified genes involved in auxin biosynthesis, transport and response, nutrient uptake and stem cell signaling. Graduate student projects are available in multiple areas, all driven by our goal of understanding how meristems give rise to organs.
Project 1) The McSteen lab is best known for research on the plant growth hormone, auxin. See http://maizeauxre.missouri.edu/ for more information on our current NSF Plant Genome project. Our long-term goal is to understand how such a simple molecule as auxin has such diverse developmental effects in different tissues.
Project 2) The McSteen lab is currently funded by an internal grant to study the intersection between meristem development and environmental stress – in particular, nutrient deficiency. We have collaborative projects on the role of boron and iron in maize root development.
Project 3) The McSteen lab is just starting a collaborative project on vesicle trafficking in maize. We have identified multiple mutants defective in trafficking through their effects on auxin transport and plan to develop tools to study the role of vesicle trafficking in development, nutrient uptake and immunity.
Project 4) The McSteen lab also has an “EvoDevo” project on the evolution of inflorescence architecture in the grasses. Our graduate student on this project recently graduated so we have openings to pick up this project at a very exciting stage.
The lab is characterized by its team approach to maize genetics. Team McSteen is currently made up of a graduate student, a post-bac student and three undergraduate students. Check out some lab pictures at https://www.instagram.com/teammcsteen/
Research in our lab focuses on vertebrate musculoskeletal form, ontogeny, and function in the contexts of animal feeding, locomotion, and evolution. We integrate studies across multiple scales from micro-scale structural materials of bone to macro-scale whole bone morphology to evolutionary changes across vertebrate taxa and with comparisons to extinct organisms.
Recently, we are most often working on computational projects aimed at developing tools and techniques for visualizing 3D imaging and complex data sets.
Craniofacial Growth. For orthodontic and craniofacial surgical interventions, estimation of the timing of peak growth and/or the amount of remaining growth is important for planning treatment. We are seeking to estimate these quantities for a wide range of traits in two- and three-dimensional imaging. We collaborate with Richard Sherwood (University of Missouri Pathology and Anatomical Sciences), Dana Duren (University of Missouri Department of Orthopaedic Surgery), Kieran McNulty (University of Minnesota), Heesoo Oh (University of the Pacific), and Manish Valiathan (Case Western Reserve University). The overall goal is to understand the patterns of human craniofacial growth and variation. There are opportunities for developing methods for studying human growth, nonlinear modelling of growth, and 2D and 3D geometric morphometrics.
New Approaches to Muscle Architecture and Function. To study the evolution of feeding, grasping, and locomotion, it is important to be able to accurately estimate muscle forces and their direction of action. In collaboration with Carol Ward and Casey Holliday (University of Missouri Department of Pathology and Anatomical Sciences), we are using contrast-enhanced CT imaging to explore the internal architecture of muscles in exceptional detail. Presently our studies are focused on feeding systems in archosaurs and the hands and feet of mammals. There are opportunities to learn about muscle form and function, 3D imaging, animation and modelling, and to develop tools for visualizing and quantifying muscle architecture.
We are broadly interested in the variable rate of life history evolution among populations and species. We focus on understanding the molecular mechanism underlying differential rates of aging, and how aging influences or is influenced by other life history traits. We use systems approaches to follow the flow of genetic information from genes to affect the pace of aging, and how environmental factors modulates the rate of genetic change in return. Using a combination of field observations, semi-controlled setups, and laboratory experiments, we aim to discover new processes that play into the aging process in the wild, and potentially derive novel testable hypotheses.
We seek graduate students who are interested in understanding aging from an ecological-evolutionary perspective. Our working hypothesis is that aging proceeds somewhat differently in free-living populations (compared to inbred populations), and that investigating aging in natural populations should uncover new molecular players in the process. Students will work with the African turquoise killifish and fruit flies to integrate inquiry across scales of biological organization, across species/populations, and across environmental conditions. There is opportunity to grow skills in the wet lab (whole-genome and transcriptome sequencing and analysis) and bioinformatic/modeling aspects of systems biology.
We are an integrative neurobiology lab broadly interested in how the brain generates healthy electrical activity, and in turn, how neural circuits acquire energy to support their synaptic and cellular functions. These interests fall into two main projects. First, we discovered that the frog brain shifts into a state that does not need oxygen and glucose to function. We are trying to understand the cellular, molecular, and metabolic mechanisms that allow neural circuits to reduce their energy demands but maintain seemingly normal function during conditions that resemble a stroke. Second, we study the neural processes that allow frogs to restart breathing after hibernating underwater for several months. Inactivity in neuromuscular systems usually leads to neurological issues. Thus, frog brains appear to have “figured out” how to avoid these problems. Although we work in comparative animal models, this work has implications for the discovery of novel treatments of stroke and neuronal injury, as we are uniquely positioned discover new forms of plasticity that improve fitness of neural circuits.
To answer these questions, we use a “genes to behavior” approach. This provides students with integrative training in diverse areas that include molecular biology, cellular neuroscience, and whole-animal physiology. We welcome students with experience in molecular methods and bioinformatics who wish to apply those skills to questions in neurophysiology. Likewise, we welcome students with a passion for animal physiology and behavior but would like to connect those processes to cellular and molecular mechanisms.
The Schulz Lab focuses on how plasticity and stability are balanced in individual neurons and neural networks by studying natural network outputs as well as responses to injury and removal of network inputs. Using a combination of single-cell electrophysiology and molecular biology techniques, we study regulation of channel and receptor proteins and how this influences neuronal excitability. Using this approach, we can investigate not only the effects at the single cell level, but also the influence that changes in single neurons has on the network activity as a whole. This work has implications not only for understanding how networks maintain functional output, but also what goes wrong when these networks fail, as is the case with some diseases and injuries that affect the circuitry of the brain and spinal cord.
We are interested in adding talented scholars who want to tackle these questions in a way that complements their long-term goals. We have room to delve deeply into single-cell sequencing analyses and fundamental molecular pathways involved in neuronal plasticity. We also have room for students who want to understand systems neuroscience from the “ground up,” and look at these interactions in both invertebrate models (crustaceans and leeches) as well as rodent models of injury and disease. We have ongoing work in impacts of spinal cord injury and multiple sclerosis on bladder function as well as deep mechanistic investigation of fundamentals of circuit plasticity in the crustacean stomatogastric ganglion.
We investigate how plant cells determine which fragments of DNA to “silence” the expression from. These regions of the genome that are commonly targeted for repression (“silencing”) are Transposable Elements (TEs), which are mobile ‘jumping genes’ that cause mutations when they jump. The overarching question driving our lab is how plant cells understand which regions of the genome are TEs to target silencing to.
In a broad sense, what the Slotkin laboratory aims to determine is how self-perpetuating feed-forward cycles in the cell are initiated. The cycles we work on are RNA interference (RNAi), RNA-directed DNA Methylation (RdDM) and maintenance epigenetic silencing. We have learned that these cycles all work to silence TEs, and can feed into each other. Our current goal is to determine how the first cycle of RNAi is triggered by TE transcripts.
Projects in the lab vary from proteomics to genomics, bench-work to purely computational. The majority of our research is done on the powerful reference plant Arabidopsis, while through collaborations we have also concentrated on maize, soybeans and aphids, and bioinformatically on many other genomes. Our expertise are in: Transposable Elements, Plant Epigenetics, RNAi, RdDM, small RNAs, DNA methylation and the Bioinformatics of multi-copy regions of the genome.
Visit the Slotkin Lab homepage at: https://slotkinlab.github.io
See what the lab has recently published: https://pubmed.ncbi.nlm.nih.gov/?term=slotkin+RK&sort=pubdate
Learn more about the Danforth Plant Science Center: https://www.danforthcenter.org
I will be joining the Division of Biological Sciences at the University of Missouri in Fall 2023.
Our lab seeks to understand why sexual reproduction and meiotic recombination are dominant in eukaryotic species despite their heavy evolutionary costs. To address this question, our research uses the microcrustacean Daphnia as a model system to investigate the causes and consequences of obligately asexual reproduction and the evolutionary forces driving the variation of meiotic recombination rate ad different biological scales (e.g., between populations/species). To achieve these goals, our lab currently uses a combination of high-throughput genomic and single-cell sequencing, bioinformatic genomic analysis, and CRISPR-Cas gene editing. We are looking for multiple graduate students interested in participating in the following research areas.
The genetic mechanisms underlying the origin of obligate parthenogenesis in Daphnia.
In this project, we are interested in exploring the genetic mechanisms giving rise to obligately parthenogenetic Daphnia. Our previous studies strongly suggest that the genetic incompatibility between two sexual species D. pulex and D. pulicaria is critical for the origin of obligate parthenogenesis in the hybrids of these two species.This project would be highly interesting for students who would like to combine evolutionary genomic analysis with cellular and molecular biology work to test the roles of specific genes and genetic variants in the origin of obligate parthenogenesis.
The evolution and genetic basis of meiotic recombination rate variation in Daphnia.
In this project, we are investigating the genetic variants regulating meiotic recombination rate variation across Daphnia populations/species and are examining whether meiotic recombination evolves in an adaptive fashion. To estimate recombination rate and identify variants associated with recombination rate variation, we are mainly using and developing single-sperm whole-genome sequencing methodologies and population genomic analysis. We will also use CRISPR-Cas genetic editing to modify genetic variants to examine their effect on recombination rate. This project is ideal for someone who is interested in combining their passion in evolution, single-cell sequencing, and genetic editing in one project.
CRISPR-Cas genetic editing in Daphnia.
Our lab is actively developing CRISPR-Cas gene editing methodologies for Daphnia, currently including gene knock-out, gene knock-in, and precise base editing. We have a goal of creating 1000 Daphnia mutants in the next 4-5 years and test their gene knock-out effects to gain a deeper understanding of gene function and interactions. For this research direction, we are looking for students with passion in mastering CRISPR gene editing and understanding gene functions and interactions.
How environmental mutagens affect genomic integrity.
We are interested in understanding the effect of different environmental mutagens, for example, ethyl methanesulfonate (EMS), on the mutation rate and spectrum and gene expression in Daphnia. We use mutant genomic analysis with gene knock-out mutants to understand how different genes contribute to safeguarding genome integrity against the harmful effect of mutagens. This project is desirable for students with passion in gaining a deep understanding of the interaction of environment and genomes.
Dr. Manuel Leal
Director of Graduate Studies