Effective conservation and management of species requires understanding processes across spatial and temporal scales that influence genetic diversity, distribution, abundance, and life history. Such comprehensive understanding of species’ ecology is rare, but critical for conservation decisions in the face of a rapidly changing climate and increasingly impacted landscapes. While all species face these threats, amphibians are of particular concern. In this talk, I will synthesize results and lessons learned from over a decade of research on plethodontid salamanders across spatial and temporal scales.