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Ramji Bhandari

Adjunct Assistant Professor of Biological Sciences
PhD, 2002 Hokkaido University, Japan

Office: University of North Carolina Greensboro
Phone: 336-256-0493
Additional: Website, Google-Scholar
Headshot of Ramji Bhandari


Research summary

Development of germ cells, environmental epigenetics, and transgenerational inheritance of phenotypes.

Research description

Developing germ cells are susceptible to environmental stress and consequences of exposure are both short-term and long-term. Short-term effects are limited to reproductive failure in the generation of exposed organism, whereas long-term effects are passed onto offspring across several generations in the form of both reproductive and non-reproductive diseases. Our laboratory studies epigenetic and gene expression changes associated with environmentally induced transgenerational reproductive diseases using fish and mice as experimental subjects.

Research Projects:

2015-2018: Transgenerational effects of Atrazine exposure in medaka. U.S. Geological Survey (USGS)
2014-2017: Endocrine disruption of epigenome across taxa. ONE HEALTH Project. Mizzou Advantage by the University of Missouri. (fish model)
2017-2019: Germline transmission of transgenerational epigenetic alterations to offspring induced by environmental estrogens. National Institutes of Health (NIH R21)

Select Publications

Select Publications

Smith, C.S., Vera, M.K.M., Bhandari, R.K. (2019). Developmental and epigenetic effects of Roundup and glyphosate exposure on medaka (Oryzias latipes). Aquatic Toxicology, 210:215-226. [PMID: 30875550]

Wang X, Bhandari RK. (2019). DNA methylation dynamics during epigenetic reprogramming of medaka embryo. Epigenetics. 1-10. [PMID: 31010368]

Wang X, Hill D, Tillitt DE, Bhandari, RK (2019). Bisphenol A and 17alpha-ethinylestradiol induced transgenerational differences in expression of osmoregulatory genes in the gill of medaka (Oryzias latipes). Aquatic Toxicology. 211:227-234. [PMID: 31048106]

Cleary, J.C., Tillitt, D.E., vom Saal, F.S., Bhandari, RK. (2019). Transgenerational effects of developmental Atrazine exposure on reproductive axis of medaka. (Environmental Pollution, in press).

Bhandari, R.K. (2016). Medaka as a model for studying environmentally induced transgenerational inheritance phenotypes.Environmental Epigenetics 2:1-9.

Bhandari R.K., vom Saal, F.S., Tillitt, D. E. (2015). Transgenerational effects from early developmental exposures to bisphenol A or 17α-ethinylestradiol in medaka, Oryzias latipes. Scientific Reports, 5 : 9303.

Skinner, M.K., Bhandari, R.K., Haque, M.M., Nilsson, E.E. (2015). Environmentally induced epigenetic transgenerational inheritance of altered SRY genomic binding during gonadal sex determination. Environmental Epigenetics 1: 1-10. doi: 10.1093/eep/evv004

Jandegian, C.M., Deem, S.L, Bhandari, R.K., Holliday, C.M., Nicks, D., Rosenfeld, C.S., vom Saal, F.S., Tillitt, D.E., Vélez-Rivera, V, Selcer, K.W, Yang, Y, and Holliday, D.K. (2015). Developmental exposure to bisphenol A disrupts sexual differentiation in painted turtles (Chrysemys picta). General and Comparative Endocrinology. 216:77-85.

Bhandari RK, Deem SL, Holliday DK, Jandegian CM, Kassotis CD, Nagel SC, Tillitt DE, Vom Saal FS, Rosenfeld CS (2015). Effects of the environmental estrogenic contaminants bisphenol A and 17α-ethinylestradiol on sexual development and adult behaviors in aquatic wildlife species. General and Comparative Endocrinology. 214:195-219.

Skinner MK, Guerrero-Bosagna C, Haque M, Nilsson E, Bhandari RK, McCarrey JR (2013) Environmentally induced transgenerational epigenetic reprogramming of primordial germ cells and the subsequent germ line. PLoS One. 8(7):e66318.

Paul-Prasanth B*, Bhandari RK*, Kobayashi T, Horiguchi R, Kobayashi Y, Nakamoto M, Shibata Y, Sakai F, Nakamura M, Nagahama Y. (2013). Estrogen oversees the maintenance of the female genetic program in terminally differentiated gonochorists. Sci Rep. 2013 Oct 7; 3:2862. (*Equally contributing first authors)

Bhandari, R.K., Schinke, E.N., Haque, MM, and Skinner, M.K. (2012). SRY-induced TCF21 genome-wide targets and cascade of bHLH factors during Sertoli cell differentiation and male sex determination in the rat. Biology of Reproduction, 87: 131-136.

Bhandari, R.K., Haque, M.M., and Skinner, M.K. (2012). Genome-wide analysis of downstream targets of male sex determining factors, SRY and SOX9 in the rat testis. PLoS One, 7(9): e43380.

Bhandari, R.K., Clement, T., Sadler-Riggleman, I., and Skinner, M.K. (2011). Basic helix-loop- helix transcription factor Tcf21 is a downstream target of male sex determining gene Sry. PLoS One, 6(5): e19935. pp1-11.

Clement, T*., Bhandari, R.K.*, Riggleman, I., and Skinner, M.K. (2011). Sry directly regulates Neurotrophin-3 promoter during male sex determination and testis development. Biology of Reproduction. 85: 277-284. (*Equally contributing first authors).

Bhandari, R.K., Alam, M.A., Higa, M. Soyano, K., Nakamura, M. (2005). Evidence that the estradiol-17β regulates the sex change of honeycomb grouper Epinephelus merra), a protogynous hermaphrodite fish. Journal of Experimental Zoology 330A: 497-503.

Bhandari, R.K., Higa, M., Nakamura, S. and Nakamura, M. (2004). Aromatase inhibitor induces complete sex change in a protogynous honeycomb grouper, Epinephelus merra. Molecular Reproduction and development, 67(3): 303-307.

Bhandari, R.K. Komuro, H., Nakamura, S., Higa, M., and Nakamura (2003). Gonadal restructuring and correlative steroid hormone profiles during natural sex change in protogynous honeycomb grouper, Epinephelus merra. Zoological Science 20: 1399-1404.

Honors & Awards

Selected honors and awards

Thomas Undergraduate Research Mentor Award, University of North Carolina Greensboro 2019

Bernard-Glickman Dean’s Professor, University of North Carolina Greensboro 2018

Outstanding Postdoctoral Presentation Award | CRB | Washington State University 2011

Lalor Foundation Merit Award | Society for Study of Reproduction 2011

Lalor Foundation Merit Award | Society for Study of Reproduction 2008

Young Researcher Award | Zoological Society of Japan 2007