Adjunct Professor of Biological Sciences
PhD, 1992 Rutgers University
|Office:||N131 4011 Discovery Drive|
Generation and characterization of animal models to study genetics and disease
Research descriptionOur laboratory takes a comparative medicine approach to studying human disorders by using a variety of animal models of disease. The current emphasis in the lab is on the study of polycystic kidney disease, hereditary deafness, and inflammatory bowel disease. Using both rodent and zebrafish models, we are interested in characterizing disease-causing genes and their protein products in order to elucidate the molecular pathways in which these genes/proteins participate. This knowledge will allow a better understanding of both normal and abnormal development and may ultimately lead to targeted therapeutics.
Additionally, we are interested in the generation and characterization of new animal models. Our lab has been involved in efforts to characterize rat embryonic stem cells (ESCs), isolate new rat ESC lines, create and validate a novel rat and zebrafish cell ablation model system and use evolving technologies such as CRISPR/Cas9 to create new genetically engineered rat models. We are currently studying the role of the microbiome on animal model phenotypes, including its effect in zebrafish models of stress and anxiety.
Bryda, E.C., LaVail, M.M. Letter to the editor announcing the availability of RCS and transgenic rats with P23H and S334ter rhodopsin mutations with inherited retinal degenerations (2019) Experimental Eye Research, 178, p. 176. DOI: 10.1016/j.exer.2018.10.003
Li, S., Lan, H., Men, H., Wu, Y., Li, N., Capecchi, M.R., Bryda, E.C., Wu, S. Derivation of transgene-free rat induced pluripotent stem cells approximating the quality of embryonic stem cells (2017) Stem Cells Translational Medicine, 6 (2), pp. 340-351.
Walters, E.M., Wells, K.D., Bryda, E.C., Schommer, S., Prather, R.S. Swine models, genomic tools and services to enhance our understanding of human health and diseases (2017) Lab Animal, 46 (4), pp. 167-172.
Shimoyama, M., Smith, J.R., Bryda, E., Kuramoto, T., Saba, L., Dwinell, M. Rat genome and model resources (2017) ILAR Journal, 58 (1), art. no. ilw041, pp. 42-58.
Feng, D., Dai, S., Liu, F., Ohtake, Y., Zhou, Z., Wang, H., Zhang, Y., Kearns, A., Peng, X., Zhu, F., Hayat, U., Li, M., He, Y., Xu, M., Zhao, C., Cheng, M., Zhang, L., Wang, H., Yang, X., Ju, C., Bryda, E.C., Gordon, J., Khalili, K., Hu, W., Li, S., Qin, X., Gao, B. Cre-inducible human CD59 mediates rapid cell ablation after intermedilysin administration (2016) Journal of Clinical Investigation, 126 (6), pp. 2321-2333.
Hanson, M.M., Liu, F., Dai, S., Kearns, A., Qin, X., Bryda, E.C. Rapid conditional targeted ablation model for hemolytic anemia in the rat (2016) Physiological Genomics, 48 (8), pp. 626-632.
Davis, D.J., Bryda, E.C., Gillespie, C.H., Ericsson, A.C. 16S rRNA amplicon sequencing dataset for conventionalized and conventionally raised zebrafish larvae (2016) Data in Brief, 8, pp. 938-943.
Davis, D.J., Bryda, E.C., Gillespie, C.H., Ericsson, A.C. Microbial modulation of behavior and stress responses in zebrafish larvae (2016) Behavioral Brain Research, 311, pp. 219-227.
Davis, D.J., Doerr, H.M., Grzelak, A.K., Busi, S.B., Jasarevic, E., Ericsson, A.C., Bryda, E.C. Lactobacillus plantarum attenuates anxiety-related behavior and protects against stress-induced dysbiosis in adult zebrafish (2016) Scientific Reports, 6, art. no. 33726,
Lan, H., Li, S., Guo, Z., Men, H., Wu, Y., Li, N., Bryda, E.C., Capecchi, M.R., Wu, S. Efficient generation of selection-gene-free rat knockout models by homologous recombination in ES cells (2016) FEBS Letters, pp. 3416-3424.
Hansen, S.A., Hart, M.L., Busi, S., Parker, T., Goerndt, A., Jones, K., Amos-Landgraf, J.M., Bryda, E.C. Fischer-344 Tp53-knockout rats exhibit a high rate of bone and brain neoplasia with frequent metastasis (2016) DMM Disease Models and Mechanisms, 9 (10), pp. 1139-1146.
Davis, D.J., Klug, J., Hankins, M., Doerr, H.M., Monticelli, S.R., Song, A., Hagan, C.E., Bryda, EC. Clove Oil Reduces Serum Cortisol Levels During Euthanasia and Increases Blood Collection Efficiency in Danio rerio. JAALAS 54:564-567 (2015).
Ericsson, A.C., Akter, S., Hanson, M.M., Busi, S.B., Parker, T.W., Schehr, R.J., Hankins, M.A., Ahner, C.E., Davis, J.W., Franklin, C.L., Amos-Landgraf, J.M., Bryda E.C. Differential Susceptibility to Colorectal Cancer due to Naturally Occurring Gut Microbiota. Oncotarget 6:33689-33704 (2015).
Taskiran, E.Z., Korkmaz, E., Gucer, S., Kosukcu, C., Kaymaz, F., Koyunlar, C., Bryda, E.C., Chaki, M., Lu, D., Vadnagara, K., Candan, C., Topaloglu, R., Schaefer, F., Attanasio, M., Bergmann, C., Ozaltin, F. Mutations in ANKS6 Cause a Nephronophthisis-Like Phenotype with ESRD. J Am Soc Nephrol. 25:1653-1661 (2014).
Bock, A.S., Leigh, N.D., Bryda, E.C. Effect of Gsk3 inhibitor CHIR99021 on aneuploidy levels in rat embryonic stem cells. In Vitro Cell Dev Biol Anim. 50:572–579 (2014).
Men, H. and Bryda, E.C. Derivation of a Germline Competent Transgenic Fischer 344 Embryonic Stem Cell Line. PLoS One 8:e56518 (2013).
Men, H., Bauer, B.A., Bryda, E.C. Germline Transmission of a Novel Rat Embryonic Stem Cell Line Derived From Transgenic Rats. Stem Cells and Development 21:2606-2612 (2012).
Bryda, E.C., Johnson, N.T., Ohlemiller, K.K., Besch-Williford, C.L., Moore, E., Bram, R.J. Conditional Deletion of Calcium-modulating Cyclophilin Ligand Causes Deafness in Mice. Mamm Genome 23:270-276 (2011).
Hillhouse A., Taylor J., Bryda E.C., Myles M. and Franklin C.F. Identification of quantitative trait loci on mouse Chromosome 3 and 17. Mamm Genome 22:544–555 (2011).
McElroy J.A., Bryda E.C., McKay, S.D., Schnabel R.D., Taylor J.F. Genetic Variation at a Metallothionein 2A Promoter Single Nucleotide Polymorphism in White and Black females. Journal of Toxicology and Environmental Health, Part A 73:1283-1287 (2010)
Bouvrette, D.J., Sittaramane, V., Heidel, J.R., Chandrasekhar, A., Bryda, E.C. Knockdown of Bicaudal C in Zebrafish (Danio rerio) Causes Cystic Kidneys: A Non-Mammalian Model of Polycystic Kidney Disease. Comparative Medicine 60:96-106 (2010).
Bryda, E.C. and Bauer, B.A. Chapter 20: A Restriction Enzyme-PCR-Based Technique to Determine Transgene Insertion Sites. In “Methods in Molecular Biology, Rat Genomics: Gene identification, Functional Genomics and Model Applications.” I. Anegon (ed). Humana Press, Totowa, NJ. Methods Mol Biol. 597:287-299 (2010).
Black, J., Dykes, A., Thatcher, S., Brown, D., Bryda, E.C., Wright, G.L. FRET Analysis of Actin/Myosin Interaction in Contracting Aortic Smooth Muscle. The Canadian Journal of Physiology and Pharmacology 87: 327-336 (2009).
Stagner, E.E., Bouvrette, D.J., Cheng, J., Bryda, E.C. The Polycystic Kidney Disease-related Proteins Bicc1 and SamCystin Interact. Biochemical and Biophysical Research Communications 383: 16-21 (2009).
Bryda, E.C. and Riley, L.K. Multiplex Microsatellite Markers Panels for Genetic Monitoring of Common Rat Strains. Journal of the American Association for Laboratory Animal Science 47: 37-41 (2008).
Bouvrette, D.J., Price, S.J., Bryda, E.C. The K Homology Domains of the Mouse Polycystic Kidney Disease-related Protein, Bicaudal-C (Bicc1) Mediate RNA Binding In Vitro. Nephron Experimental Nephrology 108:27-34 (2008).
Bryda, E.C., Pearson, M., Agca, Y., Bauer, B.A. Method for Detection and Identification of Multiple Chromosomal Integration Sites in Transgenic Animals Created with Lentivirus. Biotechniques 41:715-719 (2006).
Cogswell, C., Price, S. J., Hou, X., Guay-Woodford, L.M., Flaherty, L., Bryda, E. C. Positional cloning of the jcpk/bpk locus of the mouse. Mammalian Genome 14:242-249 (2003).
Bryda, E.C., Kim, H.J., Legare, M.E., Frankel, W., Noben-Trauth, K. High resolution genetic and physical mapping of modifier-of-deafwaddler (mdfw) and waltzer (v). Genomics 73: 338-342 (2001).
Di Palma, F., Holme, R.H., Bryda, E.C., Belyantseva, I.A., Pellegrino, R., Kachar, B., Steel, K.P., Noben-Trauth, K. Mutations in Cdh23, encoding a new type of cadherin, cause stereocilia disorganization in waltzer, the mouse model for Usher syndrome type 1D. Nature Genetics 27: 103-107 (2001).
Bolz, H., von Brederlow, B., Ramirez, A., Bryda, E.C., Kutsche, K., Nothwang, H-G, Seeliger, M., Cabrera, M. d.C-S., Vila, M.C., Molina, O.P., Gal, A., Kubisch, C. Mutation of CDH23, encoding a new member of the cadherin gene family, causes Usher syndrome type 1D. Nature Genetics 27:108-112 (2001).
Honors & Awards
Selected honors and awards
Induction, Phi Zeta (honor society for veterinary medicine) 2012
Recipient, Marshall University Distinguished Artist and Scholar Award for Junior Faculty 2001
Recipient, Marshall University Sigma Xi Researcher of the Year 2001
Recipient, AWIS-WV Career Enhancement Award 1998
Recipient, National Kidney Foundation Fellowship 1994
Recipient, Charles and Joanna Busch Predoctoral Fellowship 1990