Assistant Professor of Biological Sciences
PhD, 2009 Weizmann Institute of Science
|Office:||371D Christopher S. Bond Life Sciences Center|
The genetic and metabolic control of seed amino acids’ composition
Research descriptionStaple crop seeds, which are a major source of amino acids (AA) for both food and feed, are deficient in several essential amino acids. Attempts to use classical breeding or transgenic approaches to increase their levels often result in poor seed quality and germination vigor. This might be due to the fact that, despite having well characterized biochemical pathways, little is known about the AA network developmental constraints, interactions, response to environmental change, and the overall contribution to plant fitness and adaptation.
My primary research aims are to uncover the metabolic and genetic mechanisms driving the AA network’s response to multiple cellular demands and to environmental changes as well as to understand the evolutionary forces and developmental constraints that shaped them. To this end, my lab is focused on dissection of the genetic architecture that underlies the natural variation of seed AA related traits across multiple species under various environmental conditions and to evaluate their evolutionary context.
To achieve these goals, we employ GWAS and classical linkage mapping combined with molecular and genetic approaches, functional genomics, and bioinformatics. My lab’s long-term goals are to be able to model the genetic and metabolic response of the AA network to different abiotic stresses.
This information will provide the basis for genetic improvement through both transgenic approaches and classical breeding programs to ensure sustainable high quality seed.
Smith, S.D., Angelovici, R., Heyduk, K., Maeda, H.A., Moghe, G.D., Pires, J.C., Widhalm, J.R., Wisecaver, J.H. The renaissance of comparative biochemistry (2019) American Journal of Botany, 106 (1), pp. 3-13. DOI: 10.1002/ajb2.1216
Yobi, A., Batushansky, A., Oliver, M.J., Angelovici, R. Adaptive responses of amino acid metabolism to the combination of desiccation and low nitrogen availability in Sporobolus stapfianus (2019) Planta, 249 (5), pp. 1535-1549. DOI: 10.1007/s00425-019-03105-6
Li, M., An, H., Angelovici, R., Bagaza, C., Batushansky, A., Clark, L., Coneva, V., Donoghue, M.J., Edwards, E., Fajardo, D., Fang, H., Frank, M.H., Gallaher, T., Gebken, S., Hill, T., Jansky, S., Kaur, B., Klahs, P.C., Klein, L.L., Kuraparthy, V., Londo, J., Migicovsky, Z., Miller, A., Mohn, R., Myles, S., Otoni, W.C., Pires, J.C., Rieffer, E., Schmerler, S., Spriggs, E., Topp, C.N., Van Deynze, A., Zhang, K., Zhu, L., Zink, B.M., Chitwood, D.H. Topological data analysis as a morphometric method: Using persistent homology to demarcate a leaf morphospace (2018) Frontiers in Plant Science, 9, art. no. 553, DOI: 10.3389/fpls.2018.00553
Ren, Y., Yobi, A., Marshall, L., Angelovici, R., Rodriguez, O., Holding, D.R. Generation and evaluation of modified opaque-2 popcorn suggests a route to quality protein popcorn (2018) Frontiers in Plant Science, 871, art. no. 1803, . DOI: 10.3389/fpls.2018.01803
Li, A., Jia, S., Yobi, A., Ge, Z., Sato, S.J., Zhang, C., Angelovici, R., Clemente, T.E., Holding, D.R. Editing of an alpha-kafirin gene family increases digestibility and protein quality in sorghum (2018) Plant Physiology, 177 (4), pp. 1425-1438. DOI: 10.1104/pp.18.00200
Yobi, A., Angelovici, R.A High-Throughput Absolute-Level Quantification of Protein-Bound Amino Acids in Seeds (2018) Current protocols in plant biology, 3 (4), p. e20084. DOI: 10.1002/cppb.20084
Angelovici, R., Batushansky, A., Deason, N., Gonzalez-Jorge, S., Gore, M.A., Fait, A., Dellapenna, D. Network-guided GWAS improves identification of genes affecting free amino acids (2017) Plant Physiology, 173 (1), pp. 872-886.
Gao K, Warmund M, White T, Angelovici R, *Filiz B. Mobile Image Analysis for Microscopic Images of Seeds. Microscopy and Microanalysis 2017. 23(1) 1326-1327.
Gonzalez-Jorge, S., Mehrshahi, P., Magallanes-Lundback, M., Lipka, A.E., Angelovici, R., Gore, M.A., DellaPenna, D. ZEAXANTHIN EPOXIDASE activity potentiates carotenoid degradation in maturing seed (2016) Plant Physiology, 171 (3), pp. 1837-1851.
Galili G, Avin-Wittenberg T, Angelovici R, Fernie AR. The role of photosynthesis and amino acid metabolism in the energy status during seed development. Frontier In Plant Science. 2014, 5(447).
Angelovici R, Lipka A.E, Deason N, Gonzales–Jorge S, Lin H, Cepela J, Buell R, Gore M and DellaPenna D. Genome-wide analysis of branched-chain amino acid levels in Arabidopsis seed. Plant Cell. 2013, 25(12):4827-43.
Gonzalez-Jorge S, Ha SH, Magallanes-Lundback M, Gilliland LU, Zhou A, Lipka AE, Nguyen YN, Angelovici R, Lin H, Cepela J, Little H, Buell CR, Gore MA, Dellapenna D. Carotenoid cleavage dioxygenase4 is a negative regulator of β-carotene content in Arabidopsis seeds. Plant Cell. 2013, 25(12):4812-26.
Angelovici R, Fait A, Fernie AR, Galili G. A seed high-lysine trait is negatively associated with the TCA cycle and slows down Arabidopsis seed germination. New Phytol. 2011, 189(1): 148–159.
Less H*, Angelovici R*, Tzin V*, Galili G. Coordinated gene networks regulating Arabidopsis plant metabolism in response to various stresses and nutritional cues. Plant Cell. 2011, 23(4):1264-71.
Angelovici R, Galili G, Fernie A, and Fait A. Seed desiccation: a bridge between maturation and germination. Trends In Plant Sci. 2010, 15(4):211-8 (Review).
Angelovici R*, Fait A*, Zhu X, Szymanski J, Feldmesser J, Fernie AR, and Galili G. Deciphering transcriptional and metabolic networks associated with lysine metabolism during Arabidopsis seed development. Plant Physiol. 2009, 151(4):2058-2072.
Fait A*, Angelovici R*, Less H, Ohad I, Urbanczyk-Wochniak E, Fernie AR, Galili G. Arabidopsis seed development and germination is associated with temporally distinct metabolic switches. Plant Physiol. 2006 Nov; 142(3):839-54.
Honors & Awards
Selected honors and awards
Mr. & Mrs. Shouson & Yunying-Kou Jen Junior Faculty Research Award in Biology 2020
Postdoctoral Independent Career Potential Award 2015
Chair of the Plant Metabolic Engineering Gordon Research Seminar 2013
BARD Postdoctoral Fellowship (2010-2012) 2010
May 11, 2020
Graduate student Marianne Slaten celebrated for research
Jan. 28, 2020
Keeping balanced under stress
Jan. 28, 2020
2020 Faculty Fellow Awards in Biological Sciences
June 12, 2018
Angelovici awarded NSF grant to uncover genetic basis of amino acids in seeds
Feb. 15, 2018
Undergraduate Student Profile: Braden Zink
July 23, 2017
May 30, 2017
Two biology students selected as Cherng Summer Scholars
March 17, 2017
New method pinpoints metabolic genes, boosts seed biofortification efforts
Oct. 20, 2016
Oct. 26, 2015
Sept. 30, 2015
Ruthie Angelovici joins Mizzou Biology faculty