Assistant Professor of Biological Sciences
PhD, 2005 SUNY Buffalo, NY
Molecular physiology and biophysics of voltage-gated ion channels
Research descriptionWe study the molecular mechanisms underlying the function and regulation of voltage-gated ion channels. We are also interested in the interactions between ion channels and neurotoxins isolated from venomous animals. We use molecular biology, biochemistry, electrophysiology, computational modeling, and imaging.
Salari, A., Vega, B.S., Milescu, L.S., Milescu, M. Molecular interactions between tarantula toxins and low-voltage-activated calcium channels (2016) Scientific Reports, 6, art. no. 23894,
Gupta, K., Zamanian, M., Bae, C., Milescu, M., Krepkiy, D., Tilley, D.C., Sack, J.T., Yarov-Yarovoy, V., Kim, J.I., Swartz, K.J., 2015. Tarantula toxins use common surfaces for interacting with Kv and ASIC ion channels. eLife, 4(MAY):e06774.
Kalia J*, Milescu M*, Salvatierra J, Wagner J, Klint JK, King GF, Olivera BM, Bosmans F. From Foe to Friend: Using Animal Toxins to Investigate Ion Channel Function (2014), J Mol Biol 427, 158-175
Mihailescu M, Krepkiy D, Milescu M, Gawrisch K, Swartz KJ, White S. Structural interactions of a voltage sensor toxin with lipid membranes (2014), Proc Natl Acad Sci USA 111, E5463.
Milescu M, Lee HC, Bae CH, Kim JI, Swartz KJ (2012) Opening the Shaker K+ channel with hanatoxin. J Gen Physiol 141, 203-216.
Bosmans F, Milescu M,and Swartz KJ (2011) Palmitoylation influences the function and pharmacology of sodium channels. Proc Natl Acad Sci USA 108, 20213-8.
Jung HH, Jung HJ, Milescu M, Lee CW, Lee S, Lee JY, Eu YJ, Kim HH, Swartz KJ, and Kim JI (2010) Structure and orientation of a voltage-sensor toxin in lipid membranes. Biophys J 99:638-46.
Lee S, Milescu M, Jung HH, Lee JY, Bae CH, Lee CW, Kim HH, Swartz KJ, and Kim JI (2010) Solution structure of GxTX-1E, a high-affinity tarantula toxin interacting with voltage sensors in Kv2.1 potassium channels. Biochemistry 49:5134-42.